Composite

Part:BBa_M36154:Design

Designed by: Monica Chin   Group: Stanford BIOE44 - S11   (2014-10-23)


NifA sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1539
    Illegal BglII site found at 2003
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
    Illegal NgoMIV site found at 921
    Illegal NgoMIV site found at 2044
    Illegal AgeI site found at 1801
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In designing this part, we estimated the locations of the promoter sequences within the nif cluster. This may not be entirely accurate and may include extraneous sequences.


Source

The sequences in this part come from the genome of Azotobacter vinelandii.

References